The tour was first announced in January 2024, with confirmation of the match schedule and host cities to be confirmed.
Whangarei, Auckland, Hamilton, Wellington and Christchurch have now been confirmed to host matches, with the Lions playing tour matches against a Black Ferns XV and an invitational side during the tour.
Their first Test against the Black Ferns will be in Auckland, but at Mt Smart Stadium rather than the famed Eden Park.
The remaining two Test matches will be at Sky Stadium in Wellington and One NZ Stadium in Christchurch.
"This is a hugely significant milestone for the British & Irish Lions," Lions chief executive Ben Calveley said in a statement.
"This announcement makes our first-ever tour feel closer for everyone, from fans planning their trip to players dreaming of pulling on the Lions jersey."
New Zealand Rugby's acting chief executive Steve Lancaster said the tour would be "truly special".
British & Irish Lions Women's Series fixtures:
September 4th: v Black Ferns XV, Whangarei
September 11th: First Test v Black Ferns, Auckland
September 14th: v Invitational XV, Hamilton
September 18th: Second Test v Black Ferns, Wellington
September 25th: Third Test v Black Ferns, Christchurch