Last season, Newcastle’s Carabao Cup triumph marked an end to a 70-year wait for domestic silverware and the club will now seek to secure another trophy by progressing to the quarter-final.
However, they clash with City, a side they face for the fifth time this season in all competitions and a club who are looking to bounce back from a 2-2 home draw with Nottingham Forest.
Team news
Guardiola was first asked about team news ahead of the game and commented on the fitness of 20-year-old Nico O'Reilly.
“He feels better, we will see today," Guardiola said.
On Rodri after an injury-ravaged 18 months with a troublesome ACL problem: “Finally it's happening like we wanted a long time ago, when he came back, to not have setbacks, have consistency to play, play, and play.
“Step by step, he's getting better and better. Everybody knows it.”
The FA Cup means a lot to Guardiola
Moving on from team news, Guardiola was questioned on where the FA Cup sits in his list of priorities this season after losing to Crystal Palace in the final last year.
City could become just the third team to eliminate Newcastle from both the EFL Cup and FA Cup in the same season since Wimbledon in 1987-88 and Arsenal in 2007-08, a statistic the Magpies will want to avoid.
"How many finals have we played in the last few years? How many semi-finals have we played in over the last few years? A lot. That means the FA Cup is always so important to me.
"We're going to travel to Newcastle to win the game and to get to the next round - definitely, 100%."
Guardiola and Howe are close
Finally, he opened up on his relationship with Newcastle boss Eddie Howe who he says he is close with outside of their rivalry.
Howe’s side still have a lot of work to do to secure European football for next season and will rotate for the City clash after a hard-fought win over Manchester United in midweek.
"Yeah, really good. We've never (had) dinner together, but really good. A good relationship. Some of them (managers) have relationships for a long time.
"At the end of the day, we are rivals. We have to play each other, but before and after the game it's completely different."