The tech was trialed behind the scenes in the recent FA Cup quarter-final fixtures and deemed appropriate for use in the Premier League.
Semi-automated offside technology (SAOT) was first used during the 2022 World Cup in Qatar and was initially supposed to be introduced earlier this season for England's top division.
Clubs unanimously voted for its inclusion before the current campaign, but the introduction of the technology had been delayed.
Premier League chief football officer Tony Scholes recently described the tech as "the best system, the most accurate and future-proof."
In a statement on their website, the Premier League explained: "It (SAOT) provides more efficient placement of the virtual offside line, using optical player tracking, and generates virtual graphics to ensure an enhanced in-stadium and broadcast experience for fans.
"The technology maintains the integrity of the process while enhancing the speed, efficiency, and consistency of offside decision-making.
"The Premier League has worked in collaboration with PGMOL and sports data and technology company Genius Sports to develop the new semi-automated offside technology system."
Following recent non-live trials in the FA Cup, it will now be brought to league fixtures, starting at the Etihad Stadium when Manchester City host Crystal Palace on Saturday, 12th April.