Coach Montse Tome insisted the forward's absence for the previous two games was a sporting decision and not "punishment" after the controversy over ex-football federation chief Luis Rubiales forcibly kissing her at the 2023 Women's World Cup final.
Hermoso, who was last called up to the national side in October, plays regularly for Mexican team Tigres, so her exclusion has raised questions as the trial against Rubiales has now concluded.
In February, Spain's top criminal court found Rubiales guilty of sexual assault for the kiss and fined him 10,800 euros, sparing him jail in a sentence considered lenient by feminist groups.
Real Madrid goalkeeper Misa Rodríguez is also missing from the squad, but Esther Gonzalez, Alexia Putellas, and Patri Guijarro return.
Spain will face Portugal on April 4th and April 8th in a crucial double-header for the world champion's continued presence in the tournament.
La Roja sit third in their League A Group 3 which also includes England and Belgium.