Defending champion Barbora Krejcikova was knocked out of Wimbledon when she was beaten 2-6, 6-3, 6-4 by American 10th seed Emma Navarro on Court One on Saturday.
Krejcikova, seeded 17, led by a set and a break of serve but faded badly and looked physically drained in the final set in which she required treatment from the doctor.
A distressed Krejcikova battled on but was in tears as she prepared to serve to stay in the tournament at 3-5 in the deciding set, bravely holding serve to prolong the contest.
But Navarro held her nerve in the next game to seal victory and ensure there will be a new name on the women's trophy.
Navarro, who reached the quarter-finals last year, will face 18-year-old Mirra Andreeva in the next round.