"Neymar will not travel with the delegation to Cleveland," the CBF said in a statement. "He will remain in New Jersey, undergoing physical therapy and intensifying his physical recovery program."
Brazil's all-time leading scorer is sidelined by a Grade 2 calf strain, a moderate muscle injury involving a partial tear.
The Brazilian coaching staff remain hopeful that the 34-year-old will be available for their World Cup opener against Morocco on June 13th.
The five-time World Cup winners will also face Haiti and Scotland in Group C.